Cell Proliferation Assay Kit

  • TOOLS Cell Counting (CCK-8) kit

    TOOLS Cell Counting (CCK-8) kit

    TOOLS Cell Counting (CCK-8) kit is a ready-to-use solution without premixing of components. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

  • MTT Cell Proliferation and Cytotoxicity Assay Kit

    MTT Cell Proliferation and Cytotoxicity Assay Kit

    MTT Cell Proliferation Assay Kit provides a simple method for determination of cell number using standard microplate absorbance readers. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents and screening other biologically active compounds. Several methods can be used for such determinations, but indirect approaches using fluorescent or chromogenic indicators provide the most rapid and large-scale assays.Among such procedures, the MTT assay developed by Mossman1 is still among one of the most versatile and popular assays. The MTT assay involves the conversion of the water-soluble MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to an insoluble formazan.2-4 The formazan is then solubilized, and the concentration determined by optical density at 570 nm. The result is a sensitive assay with excellent linearity up to approximately 96 cells per well.

  • TOOLS Mycoplasma Detection Kit

    TOOLS Mycoplasma Detection Kit

    TOOLS Mycoplasma Detection Kit allows for quick and reliable screening of cell cultures for mycoplasma contamination. Highly undesirable, easily acquired and notorious for an elusive onset of infection, mycoplasmas tend to alter the infected cells at a molecular level, ultimately leading to visible changes in cell morphology and growth characteristics. Timely detection of mycoplasmas in cell cultures is desirable in order to deter wide-spread contamination and save on the costly efforts of elimination. Designed to perform with high specificity and sensitivity, this Kit helps minimize false positives while ensuring coverage over 200 species/strains of mycoplasmas with a quick protocol for routine screening.

  • TOOLShinning Dual Luciferase Reporter Assay Kit

    TOOLShinning Dual Luciferase Reporter Assay Kit

    TOOLShinning Dual Luciferase Reporter Assay Kit detects gene regulation by transfecting cells with a reporter plasmid and measuring the fluorescence intensity of Luciferin substrate to reflect the level of Luciferase expression. To achieve dual luciferase reporter gene detection, firefly luciferase is detected with Luciferin as a substrate, and renilla luciferase is detected with coelenterazine as a substrate, while inhibiting the activity of Firefly luciferase. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently. Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase. The transfected cells are induced by corresponding stimulator and lysed to determine the luciferase activity. The stimulatory-inducing effect of the regulatory elements is evaluated by luciferase activity. Renilla luciferase acts as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells. Firefly luciferase catalyzes the emission of Luciferin at 560 nm, and renilla luciferase catalyzes the emission of coelenterazine at 465 nm.