cDNA Synthesis Kit & RTase

  • ToolsQuant II Fast RT Kit

    ToolsQuant II Fast RT Kit

       ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

  • ToolScript MMLV RT Kit

    ToolScript MMLV RT Kit

       ToolScript RT Kit is designed for the first strand cDNA synthesis from very tiny amount of total RNA or poly (A) +RNA for two-step RT-PCR. The kit is applied combined with PCR in detection of expression of rare genes, quantitative expression level of mRNA in extremely small number of cells and cloning the cDNA of the specific gene.

  • ToolsQuant RT Kit

    ToolsQuant RT Kit

    ToolQuant RT Kit is designed for the first strand cDNA synthesis from tiny amount of total RNA or mRNA for two-step RT-PCR. ToolQuant Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus (AMV). ToolQuant Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in E. coli. ToolQuant Reverse Transcriptase has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA.

  • TOOLS Easy Fast RT Kit

    TOOLS Easy Fast RT Kit

    TOOLS Easy Fast RT Kit is designed for fast first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). The kit contains RT Enzyme, RNase Inhibitor, Random primers, Oligo dT primer, dNTP Mixture and reaction buffer. It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

  • TOOLS Quick RT Kit

    TOOLS Quick RT Kit

    TOOLS Quick RT Kit is designed for fast first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). The kit is designed for 2-step RT-qPCR. The 5× Mix contains Buffer, dNTPs, TOOLScript Reverse Transcriptase, and RNase inhibitor. Random primers, Oligo-dT primers. It takes only 15 min (50°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

  • One step probe RT-qPCR mix

    One step probe RT-qPCR mix

    TOOLS One-Step Probe RT-qPCR Mix is specially designed to perform rapid real-time PCR using probe method (TaqMan®, Molecular Beacon, etc). The kit allows both reverse transcription and gene amplification to take place in a single tube, which avoids cross contamination between samples and improves the sensitivity of detection.
    The 25×TOOLS Enzyme Mix contains a highly efficient reverse transcriptase expressed by engineering bacteria; a further-modified hot start Taq DNA polymerase, which provides high efficiency and accuracy for the amplify reaction; and RNase inhibitor. With a special modified hydrophobic motif, this RTase exerts a significant affinity for RNA and facilitates transcription through of RNA templates, especially for templates with high GC content or complex secondary structures. This product contains appropriate ion concentration, dNTPs and PCR enhancer. It stabilizes polymerases and maintain their efficiency within whole reaction process.

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