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4x Upper Buffer
TOOLS 4X Upper Buffer consist with 0.5M Tris (pH 6.8) and 0.4% SDS at the optimal concentration for convenient and quick preparation of polyacrylamide gels for protein analysis by SDS-PAGE. Dilution of 4X buffer to a 1X solution (0.125M Tris and 0.1% SDS) can be used in the preparation of the stacking gel for SDS-PAGE.
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4x Lower Buffer
TOOLS 4X Lower Buffer consist with 1.5M Tris (pH 8.8) and 0.4% SDS at the optimal concentration for convenient and quick preparation of polyacrylamide gels for protein analysis by SDS-PAGE. Dilution of 4X buffer to a 1X solution (0.375M Tris and 0.1% SDS) can be used in the preparation of the running gel for SDS-PAGE.
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TOOLS SSC 20X buffer
TOOLS SSC 20X buffer is saline-sodium citrate (SSC) buffer which is used as a hybridization buffer, to control stringency for washing steps in protocols for Southern blotting, in situ hybridization, DNA Microarray and Northern blotting.