This kit supplies a complete set of lysis reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and tissue. The optimized reagents and protocol allow non-denatured, active proteins to be purified in less than two hours. It is sufficient for extracting 50-100 protein samples, the quantity for each sample is (5-10) x 108 cells (about 50mg).
This Membrane Protein Extraction Kit can be used to extract the membrane protein efficiently from mammal tissues and cells with optimized buffer and reagent. It can enrich the membrane protein via moderate lysis buffer. Firstly, the cell is lysed by moderate lysis buffer. The hydrophilic protein dissolved in water phase, while the hydrophobic membrane protein is soluble in decontamination phase. The extracted membrane protein can be used in the Western Blotting, Two Dimensional Electrophoresis and Enzymatic Activity Analysis etc.