The ToolQuant RT Kit is designed for first-strand cDNA synthesis from a tiny amount of total RNA or mRNA for two-step reverse transcription polymerase chain reaction (RT-PCR). ToolQuant Reverse Transcriptase is a new, unique enzyme and is different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus (AMV). ToolQuant Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in Escherichia coli. ToolQuant Reverse Transcriptase has a high affinity for RNA, which enables the efficient and sensitive reverse transcription of any template, leading to high yields of cDNA.