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TOOLS為科學家認可的台灣分子生物學試劑首選品牌，已開發出各種高品質、高性價比且供貨穩定之分生試劑產品，提供台灣生技產業與科研領域科學家最全面性的產品線。

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Sample Preparation
首頁 產品介紹 科研試劑品牌 TOOLS Sample Preparation RNA Extraction TOOLSmart RNA Extractor

TOOLSmart RNA Extractor

TOOLSmart RNA Extractor is developed based on regular nucleic acid extraction Reagent with the addition of indicator. It has better lysis ability and higher sensitivity. It could be used to isolate total RNA from samples like viruses, bacteria, plant tissue, and body fluid. It maintains the integrity of RNA while disrupting cells and dissolving cell components during sample homogenization. TOOLSmart RNA Extractor can be applied to isolate RNA from both small amounts of samples (50-100 mg tissue or 5 × 106cells) and large amounts of samples (≥1 g tissue or ≥107cells) of human, animal, plant, or bacteria, within one hour. DNA and protein contamination is eliminated thoroughly from RNA products. Isolated RNA can be used in Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection assay, and molecular cloning.

 

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產品編號
規格
DPT-BD24
100 ml (2 bottles)
產品編號
DPT-BD24
規格
100 ml (2 bottles)
Datasheet
Download
MSDS
Download

產品特色

  1. High yield, rapid purification of high-quality RNA.
  2. Purify RNA within 1 hour.
  3. Suitable for any sample type.
  4. Low sample amount limitation.
  5. Retain short RNA fragment.
  6. High sensitivity, suitable for virus RNA extraction.

實驗流程

實驗數據

Efficiency with variety of samples
 
Figure 1. RNA extracted from rat liver tissue using 1mL of TOOLSmart RNA Extractor.  Sample amount: 30mg of rat liver. 3 uL of 80 uL eluted RNA was led per lane.

Figure 2. RNA extracted from HepG2 cells using 1ml of TOOLSmart RNA Extractor.  Sample amount: 1x10^6 HepG2 cells. 3 uL of 30 uL eluted RNA were led per lane.
Figure 3. RNA extracted from Oryza sativa leaves using 1ml of TOOLSmart RNA Extractor. Sample amount: 100 mg. 3 uL of 50 uL eluted RNA were loaded per lane.
Figure 4. miRNA extracted from U937 cells (8 x 10^6 cells)
                 Lane1: 100bp marker
                 Lane2: another brand
                 Lane3: TOOLSmart RNA Extractor

參考文獻

01
Long Noncoding RNA LINC02470 Sponges
MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer
  • JOURNALCancers (Basel).
  • TIME2022
  • IF6
02
Targeting ER protein TXNDC5 in hepatic stellate cell mitigates liver fibrosis by repressing non-canonical TGFβ signalling
  • JOURNALGut.
  • TIME2021
  • IF25
03
Hydroxygenkwanin Increases the Sensitivity of Liver Cancer Cells to Chemotherapy by Inhibiting DNA Damage Response in Mouse Xenograft Models
  • JOURNALInt J Mol Sci.
  • TIME2021
  • IF5
04
Progranulin A Promotes Compensatory Hepatocyte Proliferation via HGF/c-Met Signaling after Partial Hepatectomy in Zebrafish
  • JOURNALInt J Mol Sci.
  • TIME2021
  • IF5
05
LINC01348 suppresses hepatocellular carcinoma metastasis through inhibition of SF3B3-mediated EZH2 pre-mRNA splicing
  • JOURNALOncogene.
  • TIME2021
  • IF7
06
Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats
  • JOURNALJ Orthop Translat.
  • TIME2021
  • IF5
07
High prevalence of HIV-1 transmitted drug resistance and factors associated with time to virological failure and viral suppression in Taiwan
  • JOURNALJ Antimicrob Chemother.
  • TIME2021
  • IF5
08
Simvastatin Enhances the Chondrogenesis But Not the Osteogenesis of Adipose-Derived Stem Cells in a Hyaluronan Microenvironment
  • JOURNALBiomedicines.
  • TIME2021
  • IF4
09
Nucleophosmin/B23 promotes endometrial cancer cell escape from macrophage phagocytosis by increasing CD24 expression
  • JOURNALJ Mol Med (Berl).
  • TIME2021
  • IF4
10
Ellagic Acid Resensitizes Gemcitabine-Resistant Bladder Cancer Cells by Inhibiting Epithelial-Mesenchymal Transition and Gemcitabine Transporters
  • JOURNALCancers (Basel).
  • TIME2021
  • IF6
11
Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy
  • JOURNALTheranostics.
  • TIME2021
  • IF8
12
Hyperpolyploidization of hepatocyte initiates preneoplastic lesion formation in the liver
  • JOURNALNat Commun.
  • TIME2021
  • IF12
13
2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression
  • JOURNALFront Oncol.
  • TIME2020
  • IF4
14
Synergistic effects of pazopanib and hyperthermia against uterine leiomyosarcoma growth mediated by downregulation of histoneacetyltransferase 1
  • JOURNALJ Mol Med (Berl).
  • TIME2020
  • IF4
15
Exosome-Derived LINC00960 and LINC02470 Promote the Epithelial-Mesenchymal Transition and Aggressiveness of Bladder Cancer Cells
  • JOURNALcells.
  • TIME2020
  • IF5
16
Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells
  • JOURNALFront Oncol.
  • TIME2020
  • IF4
17
Suppression of Hepatocellular Carcinoma Progression through FOXM1 and EMT Inhibition via Hydroxygenkwanin-Induced miR-320a Expression.
  • JOURNALBiomolecules.
  • TIME2019
  • IF4
18
Psorachromene Suppresses Oral Squamous Cell Carcinoma Progression by Inhibiting Long Non-coding RNA GAS5 Mediated Epithelial-Mesenchymal Transition
  • JOURNALFront Oncol.
  • TIME2019
  • IF4
19
Palbociclib Promotes Dephosphorylation of NPM/B23 at Threonine 199 and Inhibits Endometrial Cancer Cell Growth
  • JOURNALCancers (Basel).
  • TIME2019
  • IF5
20
CPEB2‐dependent translation of long 3′‐UTR Ucp1 mRNA promotes thermogenesis in brown adipose tissue
  • JOURNALEMBO J.
  • TIME2018
  • IF10

常見問題

Q1 RNA Extractor (200ml) 共可以進行多少組織的RNA萃取?

A1 請參考Data Sheet使用，平均1ml RNA Extractor可以抽取20~50mg的組織

Q2 RNA Extractor是否可以萃取miRNA?

A2 可以，RNA Extractor可以萃取所有片段大小的Total RNA (miRNA)

Q3 若是同時要抽脂肪、肌肉、肝、細胞等組織的RNA Extraction是否有合適的kit?

A3 若要同時符合這麼多種組織使用(由其脂質)，建議還是選擇Smart Extractor比較好

Q4 請問目前有沒有推薦的exosome RNA extraction kit?

A4 TOOLSmart RNA Extractor可以萃取exosome RNA
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