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TOOLS

TOOLS為科學家認可的台灣分子生物學試劑首選品牌,已開發出各種高品質、高性價比且供貨穩定之分生試劑產品,提供台灣生技產業與科研領域科學家最全面性的產品線。

TOOLSite-Directed Mutagenesis Kit

TOOLSite-Directed Mutagenesis Kit is designed for rapid site-directed mutagenesis based on new rapid cloning technology. The amplified target fragments are digested by DpnI, cyclized by Toolsitase/buffer, and then transformed to E. coli to finish the site-directed mutagenesis. The kit consists of two modules: TOOLS Ultra High Fidelity DNA Polymerase amplification module and rapid cloning module. The ultra-fidelity of TOOLS Ultra High Fidelity DNA Polymerase can significantly reduce the possibility of unexpected mutations during amplification of up to 20 kb in length. The rapid cloning module allows homologous recombination reactions to replace the conventional annealing ring-forming reactions, requiring much less template and more flexible primer design strategy. Due to the efficient seamless splicing of the two PCR products by Toolsitase technology, the kit can complete the two separate sites mutation by single amplification reaction. DpnI digested products of the specific amplicons can be directly added into recombination reaction without purification. Highly optimized reaction buffer, fast operating procedures and amazing site-directed mutagenesis efficiency make TOOLSite-Directed Mutagenesis Kit the preferred kit for DNA site mutation.

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產品編號
規格
TTC-CB14
10 rxns.
產品編號
TTC-CB14
規格
10 rxns.
Datasheet
MSDS
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