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HE Swift Cloning Kit
TOOLS HE Swift Cloning Kit is an advanced positive selection system for the highest efficiency cloning of PCR products generated with Pfu DNA polymerase, Taq DNA polymerase, or other thermo-stable DNA polymerases. Additionally, any other DNA fragment, either blunt or sticky-end, can be successfully cloned using the kit. Cloning is fast and efficient; ligation takes only 5 minutes and yields more than 99% positive clones.
The kit features the novel positive selection cloning vector pHE. This vector contains a lethal gene which is disrupted by ligation of a DNA insert into the cloning site. As a result, only cells with recombinant plasmids are able to propagate, eliminating the need for expensive blue/white screening.
The vector contains an expanded multiple cloning site, as well as a T7 promoter for in vitro transcription. Sequencing primers are included for convenient sequencing of the insert.
Cloning Principle
Quick and efficient: Blue/white screening is not necessary, and the whole ligation process can be finished within 5 min.
Sensitive and widely applied: Good for the ligation of low concentration fragments and long fragments, works for both blunt and sticky end product.
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TOOLS Easy SYBR qPCR Mix
TOOLS Easy SYBR qPCR Mix kit is specially designed to perform Real-time PCR in SYBR Green I fluorescent-based detection assays. The Real-Time PCR reaction buffer, a 2× pre-mixed solution included in this kit, provides an optimum concentration of SYBR Green I solution, which greatly facilitates the preparation of qPCR reaction mixture. TOOLS 2xSYBR PreMix adopts a unique dual hot-start enzymes system (chemically modified HotStar Taq DNA polymerase and antibody modified Anti Taq DNA Polyerase), which, plus the pre-optimized buffer solution, provides a convenient format for highly sensitive and specific qPCR amplification.
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TOOLS Easy Fast RT Kit
TOOLS Easy Fast RT Kit is designed for fast first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). The kit contains RT Enzyme, RNase Inhibitor, Random primers, Oligo dT primer, dNTP Mixture and reaction buffer. It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.
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EasyPrep EndoFree Maxi Plasmid Extraction kit V2
TOOLS Endotoxin Free Maxi Plasmid kit V2 is based on alkaline lysis technology followed by adsorption of DNA onto silica membrane in the presence of high salt. This product is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and transformation, in vitro translation, and transfection of robust cells. With endotoxin removal buffer, the residual endotoxin is as low as <0.1 EU/μg.
Recommended bacterial culture volume: 500~1500μg plasmid with 100ml bacterial culture for high-copy vectors; and 50~300μg plasmid with 200ml bacterial culture for low- copy vectors. -
EasyPrep Polyphenol Plant DNA Extraction Kit
EasyPrep Polyphenol Plant DNA Extraction Kit provides a fast, simple, and cost-effective genomic DNA extraction method for routine molecular biology laboratory applications. The Kit uses silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. The kit is ready for use and can purify the genomic DNA from a wide variety of plant species and tissues with polyphenol contents, and the whole process is completed in less than 1 hour. Purified DNA is suitable for PCR, restriction endonuclease digestion and Southern Hybridization.
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TOOLS Cell Free DNA (cfDNA) Kit
TOOLS Cell Free DNA Kit is based on silica membrane technology and provides special buffer system. Genomic DNA binds to the silica-membrane in the presence of high salt, while the contaminants pass through the column. After the membrane is thoroughly washed to remove an remaining contaminants, the pure DNA is eluted from the membrane with low salt buffer. The kit is used for isolation DNA from serum/plasma. Purified genomic DNA can directly serve as templates for PCR, restriction enzyme digestion, hybridization, etc.
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TOOLS Buccal Swab and Saliva DNA Kit
TOOLS Buccal Swab and Saliva DNA Kit use unique silica membrane technology and special buffer system for purification of gDNA (0.5-3.5 μg) effectively from buccal/pharyngeal swab and saliva. The Spin Column CB2 made of new type silica-gel membrane in this kit can be easily bounded by DNA specifically. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either distilled water (pH 7.0-8.5) or a buffer provided with the kit. The genomic DNA isolated with the product possess high quality and can serve as an excellent template for agarose gel analysis, restriction enzyme digestion, PCR analysis and southern hybrization etc.
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小鼠基因編輯(CRISPR/Cas9 system)服務
圖爾思生物科技公司為台灣最早開始推展CRISPR/Cas9技術且為最先開始利用此系統提供客製化的基因編輯小鼠與大鼠。我們利用in vitro transcripted sgRNA與獨有的Cas9 protein，在單一個受精卵時期用microinjection方式將材料打入，六至八週九可以產出F0小鼠。
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蛋白質定量(i-TRAQ)
i-TRAQ的全名為Isobaric tags for relative and absolute quantitation ，亦即利用同位素”標籤”將來自不同樣本的蛋白用不同的同位素標記，造成在二級質譜圖分析時不同樣本來源的蛋白會有不同的分子量，因此可以區分出不同樣本來源的蛋白，達到同時對”多個樣本”進行大規模的蛋白定量。
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非標靶代謝體學分析
非標靶代謝體學 (Untargeted Metabolomics) 是藉由比較對照組和實驗組的代謝物，以尋找其代謝路徑差異的一種方法。這些差異有助於尋找臨床生物標記，應用在疾病的早期診斷或藥物的靶點發現及疾病的致病機轉研究等。
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微生物體16S擴增子定序 (16S Amplicon Sequencing)
16S rRNA為原核生物核糖體小亞基的重要組成，序列包含數個保守區域和9個高變區域，其中高變區具有屬或種的特異性，被認為是最適於細菌系統發育和分類鑑定的指標。因此對該區域中的序列進行定序已是研究環境微生物多樣性及群落組成差異的重要策略之一。
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RNA Sequencing (Quantification)
此項RNA定序技術是針對樣本中所有mRNA做高通量定序分析，通常定序結果的涵蓋率(coverage)和深度(depth)是研究人員最關心的兩樣條件。mRNA定序的結果可以顯示基因的轉錄表現量。
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