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ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.
TOOLS Cell Counting (CCK-8) kit is a ready-to-use solution without premixing of components. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.
TOOLS UltraFast PCR cloning kit is designed for fast and directional cloning of the single or multiple DNA into any vector at any site, regardless of DNA fragment end compatibility. This kit is applicable for efficient clone of fragments of 50 bp - 10 kb. The vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp - 20 bp on both 5’- and 3’-end, respectively, are mixed in an appropriate ratio and incubated with recombinase 2X UltraFast Mix at 50°C for 5 - 15 min. This kit is a novel cloning kit, applicable for homologous recombination with 1 - 5 fragments. The Kit is independent of DNA ligase, significantly reducing the self-ligated colonies. The enhanced recombinase and highly optimized buffer included in 2X UltraFast Mix significantly improve the recombination efficiency and the tolerance to impurities. pFAST vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.
i-TRAQ的全名為Isobaric tags for relative and absolute quantitation ，亦即利用同位素”標籤”將來自不同樣本的蛋白質用不同的同位素標記，造成在二級質譜圖分析時不同樣本來源的蛋白會有不同的分子量，因此可以區分出不同樣本來源的蛋白質，達到同時對”多個樣本”進行大規模的蛋白定量。 這項技術最主要可以用於進行高通量的樣品間蛋白質表達差異篩選，不但可以縮短找尋蛋白質標誌的時間，同時也可以幫研究人員節省研發經費。圖爾思技術服務中心能提供全台灣獨一無二的 i-TRAQ 技術服務，從樣品製備到上機到生物訊息分析，我們都能一條龍的提供，讓您不需要再擔心環節上的問題，在台灣圖爾思專業的服務團隊值得您信任。