探針合成(Probe Synthesis)

qPCR是一種廣泛應用於基因表現量偵測的技術，其利用PCR擴增目標基因的方式，快速的取得基因表現量的結果，但實驗的過程當中常常會因為Primer專一性的問題，而其結果的判讀，造成結果的失真。
科學家為解決這個問題，因此推出了一系列即時定量的探針，如：TaqMan、Molecular Beacons等，藉由BHQ的方法改善實驗的專一性問題，當PCR過程穩定放大相同的目標基因同時，Probe會因為Polymerase酵素的特性釋放出具有螢光的化學物質，再藉由qPCR機器收取螢光亮度進而獲得基因表現量的差異結果。
為此，TOOLS推出非常完善的即時定量探針(PCR Probe)合成的服務，有多達欲200種的Probe修飾可以選擇，其中包含非螢光的硫代修飾、磷酸化修飾等，以及螢光修飾的單邊或雙邊探針，具有應用廣泛的絕佳優勢。

螢光探針-雙化學修飾簡表：

單修飾
類別	修飾	純化方式
base	dI	PAGE
	dU	PAGE
	m5C	PAGE
	m6A	PAGE
	2'-O-Methyl	PAGE
	RNA	PAGE
	3' -dT	PAGE
	DC-Br	PAGE
	LNA-X	PAGE
	5-Methyl dC	PAGE
Phosphorothioates	S	PAGE
Phosphorylation	5' PHO	PAGE
	3' PHO	PAGE
Amino Linkers	5' NH2 C6	HPLC
	3' NH2 C7	HPLC
	5' NH2 C12	HPLC
Thiol	3' SH C3	HPLC
	5' SH C6	HPLC
Biotin	5' Biotin	HPLC
	3' Biotin TEG	HPLC
Digoxigenin	5' DIG	HPLC
	3' DIG	HPLC
Fluorescence	5' Cy3	HPLC
	3' Cy3	HPLC
	5' Cy5	HPLC
	3' Cy5	HPLC
	5' FAM	HPLC
	3' FAM	HPLC
	5' HEX	HPLC
	5' TET	HPLC
	5' -FITC	HPLC
	3' -FITC	HPLC
	5' 6-JOE	HPLC
	3' 6-JOE	HPLC
	5' ROX	HPLC
	3' ROX	HPLC
	5' TAMRA	HPLC
	3' TAMRA	HPLC
	3' -HEX	HPLC
	3' -TET	HPLC
	5' Helix Flour555	HPLC
	3' Helix Flour555	HPLC
	5' Helix Flour575	HPLC
	3' Helix Flour575	HPLC
	5' Texas Red	HPLC
	3' Texas Red	HPLC
	5' Quasar 570	HPLC
	3' Quasar 570	HPLC
	5' Quasar 670	HPLC
	3' Quasar 670	HPLC
	5' Cy3.5	HPLC
	3' Cy3.5	HPLC
	5' Cy5.5NS	HPLC
	3'Cy5.5NS	HPLC
	5' Cy7NS	HPLC
	3' Cy7NS	HPLC
	5' & 3' CAL Fluor Red 590	HPLC
	5' & 3' CAL Fluor Red 610	HPLC
	5' & 3' DABCYL	HPLC
	5' & 3' AMCA	HPLC
	5' & 3' DEAC	HPLC
	5' & 3' MCA	HPLC
	5' & 3' LRB Red	HPLC
	5' & 3' CR6G	HPLC
	5' & 3' Bodi Fluor R6G	HPLC
	5' & 3' NBD-X	HPLC
	5' & 3' California Red	HPLC
	5' & 3' iFlour	HPLC
	5' -VIC	HPLC
	3' -LC RED-610	HPLC
	3' -LC RED-640	HPLC
	3' -LC RED-705	HPLC
	3' -Bodipy 493/503	HPLC
	3' -Bodipy 564/570	HPLC
	3' -Bodipy 581/591	HPLC
	3' -Bodipy 630/650	HPLC
	3' -Bodipy 650/665	HPLC
	3 -BODIPY R6G	HPLC
	5' -SIMA	HPLC
Quenchers	3' DABCYL	HPLC
	3' TAMRA	HPLC
	3' BHQ 1	HPLC
	3' BHQ 2	HPLC
	3' BHQ 3	HPLC
	3' -MGB	HPLC
	3' BBQ-650	HPLC
	3' TQ1-TQ6	HPLC
	3' -QSY 7 carboxylic acid	HPLC
	3' TQ7	HPLC
	3' eclipse	HPLC
Other modification	Spacer9	HPLC
	Azide	HPLC
	DBCO	HPLC
	Spacer(C6)	HPLC
	Spacer9	HPLC
	Spacer(C12)	HPLC
	Spacer C3	HPLC
	Spacer C18	HPLC
	dSpacer	HPLC
	Inverted Base	HPLC
	5' -Carboxy C10	HPLC
	5' -D4PA	HPLC
	5' -Acridine	HPLC
	3' ddC	HPLC
	Alexa Flour 647	HPLC
	Yakima Yellow Epoch	HPLC
	Pyrene	HPLC
中間修飾	Int NH2 dT	HPLC
	Int Biotin dT	HPLC
	Int Cy3 dT	HPLC
	Int Cy5 dT	HPLC
	Int TAMRA dT	HPLC
	Int ROX dT	HPLC
	Int FAM dT	HPLC
	Int HEX dT	HPLC
	Int Dig dT	HPLC
	Int CHCH dT	HPLC
	Int DBCO dT	HPLC
	Int Azide dT	HPLC
	Int TAMRA dT	HPLC
	Int ATTO Flour dT	HPLC
	Int BHQ1 dT	HPLC
	Int BHQ2 dT	HPLC
	Int HS-SH C6	HPLC
	Int Alexa Flour dT	HPLC
	Int Azobenzene	HPLC
	Int Ferrocene dT	HPLC
	dT- LC RED-610	HPLC
	dT- LC RED-640	HPLC
	dT- LC RED-705	HPLC
	dC-Fluorescein	HPLC
	dC-Tamra	HPLC
	dC-Amino	HPLC
	dC-Rox	HPLC
	dC-Digoxin	HPLC
	dC-FITC	HPLC
	dC-Texas Red	HPLC

雙修飾
類別	修飾	純化方式
TAMRA	5' FAM-3' TAMRA	HPLC
	5' HEX-3' TAMRA	HPLC
	5' TET-3' TAMRA	HPLC
	5' JOE-3' TAMRA	HPLC
	5' VIC-3' TAMRA	HPLC
BHQ1	5' FAM-3' BHQ1	HPLC
	5' VIC-3' BHQ1	HPLC
	5' HEX-3' BHQ1	HPLC
	5' JOE-3' BHQ1	HPLC
	5' TET-3' BHQ-1	HPLC
BHQ2	5' Cy5-3' BHQ2	HPLC
	5' Cy3-3' BHQ2	HPLC
	5' ROX-3' BHQ2	HPLC
	5' TET-3' BHQ2	HPLC
	5' JOE-3' BHQ2	HPLC
	5' HEX-3' BHQ2	HPLC
	5' TAMRA-3' BHQ2	HPLC
	5' Texas Red-3' BHQ2	HPLC
	5' Quasar 670-3' BHQ2	HPLC
	5' FAM and 3' BHQ2	HPLC
BHQ3	5' Quasar 670-3' BHQ3	HPLC
	5' CY5-3' BHQ3	HPLC
ECLIPS	5' FAM-3' ECLIPS	HPLC
	5' HEX-3' ECLIPS	HPLC
	5' TAMRA-3' Eclipse	HPLC
	5' ROX-3' Eclipse	HPLC
	5' TET-3' Eclipse	HPLC
	5' JOE-3' Eclipse	HPLC
MGB	5' FAM-3' MGB	HPLC
	5' HEX-3' MGB	HPLC
	5' TET-3' MGB	HPLC
	5' JOE-3' MGB	HPLC
	5' ROX-3' MGB	HPLC
	5' Texas Red-3' MGB	HPLC
	5' Quasar 670-3' MGB	HPLC
	5' VIC-3' MGB	HPLC
Dabcyl	5' FAM-3' DABCYL	HPLC
	5' HEX-3' DABCYL	HPLC
	5' TET-3' DABCYL	HPLC
	5' JOE-3' DABCYL	HPLC
	5' TAMRA-3' DABCYL	HPLC
	5' Texas Red-3' Dabcyl	HPLC
	5' ROX-3' Dabcyl	HPLC
	5' CY3-3' DABCYL	HPLC
	5' Cy5-3' DABCYL	HPLC
Other	5' Phos and 3' FAM	HPLC
	5' TAMRA and 3' Phos	HPLC
	5' C6-Biotin, 3' Biotin	HPLC
	5' C6-Biotin , 3' Cy5	HPLC
	5' C6-NH2 , 3' C3-Fam	HPLC
	5' C6-NH2 , 3' Cy3	HPLC
	5' Cy5 , SS ,	HPLC
	5' Cy5 , 3' C7-NH2	HPLC
	5' Cy5 , 3' SH	HPLC
	5' Dabcyl , 3' C3-Fam	HPLC
	5' DlG , 3' Digoxin	HPLC
	5' SH,3' Cy3	HPLC
	5' Fam,3' C3-Fam	HPLC
	5' TAMRA，3' C3-Fam	HPLC

1. 提供詳細且準確的欲合成序列

2. 選擇修飾及純化方式

3. 確認合成量規格

1. 達合成規格的產品(冷凍乾燥粉末)

2. 合成報告書