在設計probe時，難免會遇到目標基因較多的AT, CG片段，導致設計的probe長度過長，影響quencher抑制螢光訊號的效果，最後過高的背景值也影響實驗結果的準確性。

圖爾思技術服務中心可以提供獨家的LFN-Quenched Probe，將LFN Quencher，以雙標定在距離5’端的第九和第十鹼基之間和3’端位點，並提高實驗的準確性。

LFN-Quenched Probe優勢：

1. 有效抑制5‘端的螢光訊號與降低背景值(Low Background)

2. 降低Cq值並提高qPCR實驗的靈敏度及準確性

3. 適合用在較長的probe

4. 效能優於一般傳統的probe

LFN-Quenched Probe Dye(s)

LFN-quenched probes compare to different probes during qPCR performance. mCherry as a template DNA was analyzed over 7 sequential 10-fold dilution aliquots. Normalized fluorescence values were plotted with or without subtracting background fluorescence. (A) A LFN double-quenched FAM/LFN/LFN probe was compared to single probe FAM/BHQ by using TOOLS Easy 2xProbe qPCR Mix (TTC-QE13). (B) LFN double-quenched probe was compared to competitive double-quenched probe by using TaqMan™ Gene Expression Master Mix (4370048). The standard curves of all groups R2 value equaled to 0.999.

※服務交期：10個工作天(視數量調整)