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miRNA RT-qPCR service of Extracellular Vesicles

圖爾思提供miRNA RT-qPCR檢測服務，給予快速、高靈敏與高特異性的檢測成熟的microRNA (miRNA)。只需一次cDNA制備，即可量化分析成熟miRNA (mature miRNA)的表達。本服務當中所使用的TOOL miRNA RT-qPCR primer/probe set包含特異性primer和probe，用於檢測和定量mature miRNA。該系統使用stem-looped system進行反轉錄（RT），並通過probe-base進行qPCR，以提高miRNA檢測的準確性。該miRNA set可區分mature miRNA序列和precursor，為miRNA定量提供高效準確的方法。

服務內容

miRNA在研究中具有廣泛的應用價值，它們作為細胞間通訊的調節者和生物學過程的調節子，在許多生命科學領域發揮著重要作用。特別是在發展疾病的分子機制研究、疾病早期診斷、藥物開發以及生物標誌物鑑定等方面，因此miRNA的應用日益受到關注。
圖爾思提供自家品牌TOOLS所擁有獨特的RT-qPCR分析系統，專注於mature miRNA的分析。憑藉這一系統，我們有豐富的樣品RNA處理經驗，並提供miRNA RT-qPCR分析服務。在這項服務中，我們引入spike-in模板作為萃取和RT-qPCR的QC control，以確保實驗的可靠性。此舉不僅有助於僅定量mature miRNA，同時也排除了前趨物（Precursor）的干擾，確保結果的準確性。

服務流程：

服務特色

  1. 從樣品處理到上樣分析，圖爾思提供一站式的分析服務
  2. 高特異性、高靈敏度的miRNA primer/probe set，提供高精準度、準確性的實驗數據
  3. 超過500個經驗證後的miRNA序列可供選擇；也提供客製化miRNA序列合成服務
  4. 經嚴格確效與驗證的高通量Panel，讓您一次獲得超過16 targets以上的實驗結果

樣品需求

Extracellular vesicles
樣品允收：10^10-10^11 particles、體積0.2-0.3mL， 保存在PBS (-80度)
服務交期：10-15個工作天

Cell Pellet
樣品允收：10^6 cells 保存在-80度
服務交期：10-15個工作天

Serum/Plasma
樣品允收：體積>0.25mL， 保存在 (-80度)
服務交期：10-15個工作天
備註：如果有Heparin需額外付樣品處理費

*目前我們有超過500 sequences可以供選擇、若有其他物種、其他miRNA sequence，請洽詢專員
 

miRNA panel

我們提供不同疾病的Panel，讓您一次可以獲得超過16 targets的實驗結果，且每個Panel會提供兩個spike-in control (cel-miR-238-3p, cel-miR-39-3p)和兩個internal control (U6, SNORD48)，讓您獲得更精準的實驗數據與減少您寶貴的時間、成本！！
 
Disease Targets Target list
Colorectal cancer (CRC) 42 hsa-miR-100-5p, hsa-miR-103a-3p, hsa-miR-106b-5p, hsa-miR-10b-5p, hsa-miR-126-3p, hsa-miR-128-3p, hsa-miR-130b-3p, hsa-miR-135a-5p, hsa-miR-139-5p, hsa-miR-141-3p, hsa-miR-142-3p, hsa-miR-143-3p, hsa-miR-145-5p, hsa-miR-148a-3p, hsa-miR-149-5p, hsa-miR-155-5p, hsa-miR-15a-5p, hsa-miR-16-5p, hsa-miR-17-5p, hsa-miR-181b-5p, hsa-miR-183-5p, hsa-miR-18a-5p, hsa-miR-191-5p, hsa-miR-195-5p, hsa-miR-19a-3p, hsa-miR-200c-3p, hsa-miR-20a-5p, hsa-miR-210-3p, hsa-miR-21-5p, hsa-miR-221-3p, hsa-miR-222-3p, hsa-miR-223-3p, hsa-miR-224-5p, hsa-miR-24-3p, hsa-miR-25-3p, hsa-miR-29a-3p, hsa-miR-30a-3p, hsa-miR-31-5p, hsa-miR-497-5p, hsa-miR-92a-3p, hsa-miR-93-5p, hsa-miR-9-5p
Chronic Kidney Disease
(CKD)
 44 hsa-let-7f-5p, hsa-miR-10a-5p, hsa-miR-1207-5p, hsa-miR-125a-5p, hsa-miR-126-3p, hsa-miR-1307-5p, hsa-miR-135a-5p, hsa-miR-140-3p, hsa-miR-141-3p, hsa-miR-145-5p, hsa-miR-146a-5p, hsa-miR-17-3p, hsa-miR-182-5p, hsa-miR-192-5p, hsa-miR-194-5p, hsa-miR-195-5p, hsa-miR-19a-3p, hsa-miR-204-5p, hsa-miR-20a-5p, hsa-miR-214-3p, hsa-miR-21-5p, hsa-miR-221-3p, hsa-miR-26a-5p, hsa-miR-30d-5p, hsa-miR-3166, hsa-miR-320d, hsa-miR-342-3p, hsa-miR-34a-5p, hsa-miR-378a-5p, hsa-miR-421, hsa-miR-4260, hsa-miR-4301, hsa-miR-4304, hsa-miR-4479, hsa-miR-452-5p, hsa-miR-489-3p, hsa-miR-508-3p, hsa-miR-532-5p, hsa-miR-574-3p, hsa-miR-619-3p, hsa-miR-625-5p, hsa-miR-627-5p, hsa-miR-629-5p, hsa-miR-99b-5p
Acute Kidney Injury
(AKI)		 39  hsa-let-7f-5p, hsa-let-7g-5p, hsa-miR-107, hsa-miR-10a-5p, hsa-miR-125a-5p, hsa-miR-126-3p, hsa-miR-127-3p, hsa-miR-130b-5p, hsa-miR-135a-5p, hsa-miR-140-3p, hsa-miR-145-5p, hsa-miR-155-5p, hsa-miR-16-5p, hsa-miR-18a-5p, hsa-miR-192-5p, hsa-miR-19b-3p, hsa-miR-200c-3p, hsa-miR-204-5p, hsa-miR-205-5p, hsa-miR-210-3p, hsa-miR-214-3p, hsa-miR-21-5p, hsa-miR-23a-3p, hsa-miR-24-3p, hsa-miR-296-3p, hsa-miR-29a-3p, hsa-miR-29b-2-5p, hsa-miR-30c-5p, hsa-miR-30d-5p, hsa-miR-320a-3p, hsa-miR-324-3p, hsa-miR-324-5p, hsa-miR-451a, hsa-miR-489-3p, hsa-miR-494-3p, hsa-miR-532-5p, hsa-miR-574-3p, hsa-miR-92b-3p, hsa-miR-99a-5p
Kawasaki disease
(KD)		 16 hsa-miR-1246, hsa-miR-125a-5p, hsa-miR-140-3p, hsa-miR-145-5p, hsa-miR-148a-3p, hsa-miR-182-5p, hsa-miR-183-5p, hsa-miR-197-3p, hsa-miR-223-3p, hsa-miR-27a-3p, hsa-miR-30c-5p, hsa-miR-30e-3p, hsa-miR-378a-3p, hsa-miR-4436b-5p, hsa-miR-671-5p
Obesity 37 hsa-let-7i-3p, hsa-miR-101-5p, hsa-miR-106b-3p, hsa-miR-106b-5p, hsa-miR-10b-5p, hsa-miR-122-5p, hsa-miR-1246, hsa-miR-125a-5p, hsa-miR-127-3p, hsa-miR-128-3p, hsa-miR-1285-3p, hsa-miR-15b-3p, hsa-miR-181a-5p, hsa-miR-182-5p, hsa-miR-183-5p, hsa-miR-186-5p, hsa-miR-194-5p, hsa-miR-196a-5p, hsa-miR-25-3p, hsa-miR-30e-5p, hsa-miR-31-5p, hsa-miR-328-3p, hsa-miR-342-3p, hsa-miR-342-5p, hsa-miR-3688-3p, hsa-miR-421, hsa-miR-486-3p, hsa-miR-500a-3p, hsa-miR-502-3p, hsa-miR-532-5p, hsa-miR-548f-5p, hsa-miR-548h-5p, hsa-miR-7706, hsa-miR-877-5p, hsa-miR-92a-3p, hsa-miR-92b-3p, hsa-miR-93-5p
Epstein-Barr virus (EBV) 44 ebv-miR-BHRF1-1, ebv-miR-BART3-5p, ebv-miR-BART7-5p, ebv-miR-BART11-5p, ebv-miR-BART16, ebv-miR-BART20-3pB, ebv-miR-BHRF1-2-5p, ebv-miR-BART3-3p, ebv-miR-BART7-3p, ebv-miR-BART11-3p, ebv-miR-BART17-5p, ebv-miR-BART21-5pC, ebv-miR-BHRF1-2-3p, ebv-miR-BART4-5p, ebv-miR-BART8-5p, ebv-miR-BART12, ebv-miR-BART17-3p, ebv-miR-BART21-3pD, ebv-miR-BHRF1-3, ebv-miR-BART4-3p, ebv-miR-BART8-3p, ebv-miR-BART13-5p, ebv-miR-BART18-5p, ebv-miR-BART22E, ebv-miR-BART1-5p, ebv-miR-BART5-5p, ebv-miR-BART9-5p, ebv-miR-BART13-3p, ebv-miR-BART18-3p, ebv-miR-BART1-3p, ebv-miR-BART5-3p, ebv-miR-BART9-3p, ebv-miR-BART14-5p, ebv-miR-BART19-5p, ebv-miR-BART2-5p, ebv-miR-BART6-5p, ebv-miR-BART10-5p, ebv-miR-BART14-3p, ebv-miR-BART19-3p, ebv-miR-BART2-3p, ebv-miR-BART6-3p, ebv-miR-BART10-3p, ebv-miR-BART15, ebv-miR-BART20-5p

出貨規格

  • miRNA RT-qPCR Report
  • Raw data

相關產品

TOOLS miRNA RT Kit
Cat#TTH-mi50 / TTH-mi250

TOOLS miRNA RT Kit provides the perfect first step of TOOLS miRNA RT-qPCR assay. This Kit adopts an innovative RT enzyme and buffer system, which enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
  • Easy: Compatible with TOOLS miRNA RTqPCR primer/probe set, only common RT procedure required. 
  • Fast:  RT reaction completed only within 15 mins.
  • Accurate: Combination of an efficient reverse transcriptase and RNase inhibitor
  • Efficient: Reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template
TOOLS Easy 2xProbe qPCR Mix
Cat#TTC-QE13

This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions.  TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
  • Easy: All-in-one optimized master mix
  • Specific: Premixed with dUTP/UDG anticontamination system
  • High efficiency and stability
  • ROX Reference Dye for fluorescent signal normalization and compatible with all qPCR instruments.
EasyPrep miRNA Extraction Kit
Cat#DPT-BE01

EasyPrep miRNA Extraction Kit utilizes a silica-based system to enrich and purify small RNA, including miRNA, small interfering RNA (siRNA), small nuclear RNA (snRNA), and total RNA from various sample sources (cell, animal tissue, plant tissue, serum, plasma), especially for small RNA of <200 nt. The lysis buffer in the kit is optimized to exert excellent lysis ability and isolation efficiency. High-quality isolated product without contamination of DNA and protein could be obtained within 1 hour, and directly used in Northern Blot, Dot Blot, Poly A screening, in vitro translation, RNase protection analysis, and also molecular cloning.
  • Efficient: Completed within 1 hr
  • Quality: silica-based system to yield high-purity small RNA product
  • Flexible: Excellent lysis ability for various sample sources

參考文獻

01
Next-generation sequencing identifies micro-RNA-based biomarker panel for Kawasaki disease
  • JOURNALJournal of Allergy and Clinical Immunology
  • TIME2016
  • IF7.79
02
Serum microRNA panels predict bariatric surgery outcomes
  • JOURNALObesity (Silver Spring, Md.)
  • TIME2022
  • IF6.90
03
A circulating miRNA signature for early diagnosis of acute kidney injury following acute myocardial infarction
  • JOURNALJournal of Translational Medicine
  • TIME2019
  • IF4.124
04
MicroRNA-223 and microRNA-92a in stool and plasma samples act as complementary biomarkers to increase colorectal cancer detection
  • JOURNALOncotarget
  • TIME2016
  • IF5.168
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