TOOLS Relative Human Telomere Length qPCR Assay Kit
TOOLS Relative Human Telomere Length qPCR Assay Kit is engineered for the precise comparative evaluation of average human telomere length across diverse sample groups using a robust SYBR Green qPCR platform. By simultaneously assessing telomeric DNA repeats alongside an 84 bp single-copy reference gene (SCG) on chromosome 9, the assay accurately determines relative telomere length by calculating the T/S (Telomere-to-Single Copy Gene) ratio of experimental samples relative to a control sample.
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產品編號
規格
TTT-TL02
50 rxns
產品編號
TTT-TL02
規格
50 rxns
產品特色
- Accurate Relative Quantification：Determines relative telomere length by calculating the T/S (Telomere-to-Single Copy Gene) ratio using the 2-ΔΔCq method.
- Superior Precision & Reproducibility：Delivers exceptional batch-to-batch consistency with a low coefficient of variation (CV < 6%) and an outstanding run-to-run correlation (R2 = 0.9511) across independent assays.
- High PCR Efficiency & Wide Linearity：Reaches near-optimal amplification efficiency (TEL E = 101.2%；SCG E = 99.1%) and high linearity (R2 = 0.999) across a broad dynamic range (0.08-10 ng gDNA).
- Minimal Sample Input Requirement：Optimized for ultra-low DNA input (1-2.5 ng gDNA per reaction), preserving precious primary cells, stem cells, and blood samples.
- Clean Signal & High Specificity：Utilizes a high-performance hot-start DNA polymerase to effectively suppress primer dimers and non-specific amplification.
- Fast & High-Throughput Workflow：Completes comparative quantitative analysis in under 2 hours, offering a streamlined qPCR alternative to complex Southern blot/TRF methods.
實驗數據
- Performance of TEL Quantification：
Representative telomere amplification plots and standard curves generated from human genomic DNA ranging from 0.08 ng to 10 ng per reaction.
- Performance of SCG Quantification：
Representative SCG amplification plots and standard curves generated from human genomic DNA ranging from 0.08 ng to 10 ng per reaction.
