TOOLS EBV miRNA Multiplex RT-qPCR Assay includes designed and validated RT-qPCR primer/probe set targeting 44 respect EBV mature microRNAs (Table 1) that are suggested of crucial and potential roles in EBV related disease. This assay system utilizes a stem-looped primer to specifically extend the cDNA of target miRNA in reverse transcription (RT) and is an exclusively designed set for the probe-based qPCR assay to increase detection accuracy. Combined with TOOLSQuant II Fast RT kit and TOOLS Easy 2X probe qPCR mix, TOOLS EBV miRNA Multiplex RT-qPCR Assay System allows rapid, sensitive, and specific detection and quantification of 48 microRNAs in a total RNA sample respectively. TOOLS EBV miRNA Multiplex RT-qPCR Assay also includes the primer/probe sets for U6 and SNORD48 as the normalization controls and two exogenous miRNAs (cel-miR-39-3p, and cel-miR 238-3p) as spike-in controls