TOOLS SuperFast cDNA First-Strand Synthesis Kit
The SuperFast cDNA First-Strand Synthesis Kit is a high-efficiency, stable, and fast reverse transcription system designed to remove genomic DNA contamination. The kit contains a powerful gDNA Eraser that eliminates residual gDNA within 2 minutes at 42°C, ensuring the purity of RNA samples. This kit features a next-generation reverse transcriptase with improved RNA affinity, thermal stability, and sensitivity, making it ideal for complex RNA templates. It enhances transcription efficiency and reaction speed, offering superior performance even with high-GC content and complex secondary structures.
產品編號
規格
KRT-BA36
100 rxns.
產品編號
KRT-BA36
規格
100 rxns.
產品特色
- Super Fast：Complete synthesis cDNA in just 10 minutes.
- Sensitivity：Reliable detection down to 1ng RNA.
- Easy-to-Use：Simple mix-and-go workflow with simple step.
- Economical：Premium performance at a competitive price.
- High-Efficiency Reverse Transcription：Optimized primer mix design enables efficient full-length cDNA synthesis and robust amplification.
- Broad Downstream Compatibility：Delivering flexible integration into diverse molecular biology and gene expression applications.
實驗數據
Comparison Test：
Comparison of reverse transcription performance between SuperFast and QuantII kits.
HeLa total RNA (100, 10, and 1 ng) was reverse transcribed and ACTB (353 bp) expression was quantified using TOOLS 2× SYBR qPCR Mix (#FPT-BB05). Both kits showed comparable Ct values across all RNA inputs, indicating similar reverse transcription efficiency and sensitivity. Error bars represent technical replicates.
Consistency of PCR amplification from synthesized cDNA.
PCR products derived from cDNA were analyzed by agarose gel electrophoresis. Lane M: DNA ladder. All samples show clear single bands at the expected size with comparable band intensity, demonstrating consistent cDNA quality and reproducible amplification across reactions.
Comparison of reverse transcription performance between SuperFast and QuantII kits.
Consistency of PCR amplification from synthesized cDNA.
PCR products derived from cDNA were analyzed by agarose gel electrophoresis. Lane M: DNA ladder. All samples show clear single bands at the expected size with comparable band intensity, demonstrating consistent cDNA quality and reproducible amplification across reactions.
