TOOLSee Colorimetric RT-LAMP kit is a dual enzyme system including an optical indicator which offers a simple and one step solution to detect target gene in form of RNA via reverse transcription loop-mediated isothermal amplification (RT-LAMP) simply in a fixed temperature within 30 to 60 minutes. This kit contains optimized metal intercalating indicator of which color changes from violet to sky blue reflecting the reducing concentration of Mg⁺² for quick and easy detection of RT-LAMP reaction master mix inhibiting amplification efficiency. This kit was validated and used to detect RNA from purified nucleic sample and compatible with crude extract samples such as saliva, which makes TOOLSee Colorimetric RT-LAMP kit ideal for the detection of virus and micro pathogens. The end amplicon of TOOLSee Colorimetric RT-LAMP kit can be also verified by agarose gel electrophoresis.