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TOOLS

TOOLS為科學家認可的台灣分子生物學試劑首選品牌,已開發出各種高品質、高性價比且供貨穩定之分生試劑產品,提供台灣生技產業與科研領域科學家最全面性的產品線。

TOOLSite-Directed Multi-Mutagenesis Kit

TOOLSite-Directed Multi-Mutagenesis Kit is designed for rapid site-directed mutagenesis based on new rapid cloning technology. This kit can fulfill 3-5 separate site-directed mutagenesis in one reaction.
The amplified target fragments are digested by DpnI, cyclized by Multi-Toolsitase/buffer, and then transformed to E. coli to finish the site-directed mutagenesis. The kit consists of two modules: TOOLS Ultra High Fidelity DNA Polymerase amplification module and rapid cloning module. The ultra-fidelity of TOOLS Ultra High Fidelity DNA Polymerase can significantly reduce the possibility of unexpected mutations during amplification of up to 20 kb in length. The rapid cloning module allows homologous recombination reactions to replace the conventional annealing ring-forming reactions, requiring much less template and more flexible primer design strategy. The kit is equipped with Multi-Toolsitase, a recombinant enzyme specifically optimized for multi-sites-directed mutations. DpnI digestion of the specific amplification products can be directly applied in recombination reaction without purification. Highly optimized reaction buffer, fast operating procedures and amazing site-directed mutagenesis efficiency make TOOLSite-Directed Mutagenesis Kit the preferred kit for DNA multi-sites mutation.

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產品編號
規格
TTC-CB15
10 rxns.
產品編號
TTC-CB15
規格
10 rxns.
Datasheet
MSDS
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